bs-6480R-A350 [Conjugated Primary Antibody]
CH25H Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CH25H

Immunogen Range: 1-100/272


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9023

Swiss Prot: O95992

Source: KLH conjugated synthetic peptide derived from human CH25H

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 32


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mukherjee, Pallavi, et al. "Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-β and Cholesterol 25-hydroxylase in Jejunum." Journal of Lipid Research (2017): jlr-M076554.Read more>>
  • Chattopadhyay et al. Treating the Intestine with Oral ApoA-I Mimetic Tg6F Reduces Tumor Burden in Mouse Models of Metastatic Lung Cancer. (2018) Sci.Rep. 8:9032Read more>>
  • Samantha A. Hutchinson. et al. Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancer. Oncogene. 2021 Mar;:1-12Read more>>
  • Ceglia, Simona. et al. An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cells. NAT IMMUNOL. 2023 Jan;:1-14Read more>>
  • Tran Thanh-Tam. et al. Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss. EXP MOL MED. 2023 Dec;:1-11Read more>>
VALIDATION IMAGES