bs-6522R [Primary Antibody]
Mitochondrial Creatine Kinase Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CKMT

Immunogen Range: 115-220/417


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1159

Swiss Prot: P12532

Source: KLH conjugated synthetic peptide derived from human Mitochondrial Creatine Kinase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 43


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Matsuyama, Ryo. et al. Metabolite alteration analysis of acetaminophen-induced liver injury using a mass microscope. Anal Bioanal Chem. 2022 Mar;:1-1Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CKMT/Creatine kinase MT Polyclonal Antibody, Unconjugated (bs-6522R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti CKMT/Creatine kinase MT Polyclonal Antibody, Unconjugated (bs-6522R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CKMT/Creatine kinase MT Polyclonal Antibody, Unconjugated (bs-6522R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse small intestine lysates probed with CKMT/Mitochondrial Creatine Kinase Polyclonal Antibody, Unconjugated (bs-6522R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Large intestine tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Large intestine tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with CKMT Polyclonal Antibody, Unconjugated (bs-6522R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.