bs-6589R [Primary Antibody]
BCAT2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BCAT2

Immunogen Range: 101-200/392


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 587

Swiss Prot: O15382

Source: KLH conjugated synthetic peptide derived from human BCAT2/BCAM

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 41


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Rivera ME et al. Effect of metformin on myotube BCAA catabolism. J Cell Biochem. 2019 Aug 5.Read more>>
  • Madison E. Rivera. et al. Comparing the effects of palmitate, insulin, and palmitate-insulin co-treatment on myotube metabolism and insulin resistance. 2021 Aug 12Read more>>
  • Jason S. Hinkle. et al. AICAR stimulates mitochondrial biogenesis and BCAA catabolic enzyme expression in C2C12 myotubes. Biochimie. 2021 NovRead more>>
  • Caroline N. Rivera. et al. PPAR/ Agonism with GW501516 Increases Myotube PGC-1 Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression. PPAR RES. 2023;2023:4779199Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-BCAT2/BCAM Polyclonal Antibody, Unconjugated (bs-6589R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Mouse Kidney lysates probed with BCAT2 Polyclonal Antibody, Unconjugated (bs-6589R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.