bs-6601R [Primary Antibody]
TLR7 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TLR7

Immunogen Range: 461-560/1049


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51284

Swiss Prot: Q9NYK1

Source: KLH conjugated synthetic peptide derived from human TLR7

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 121


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Umehara T et al. Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm. PLoS Biol. 2019 Aug 13;17(8):e3000398.Read more>>
  • Umehara T et al. Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm. PLoS Biol. 2019 Aug 13;17(8):e3000398.Read more>>
  • Takashi Umeharaet al. A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed Mouseor cattle embryos. Nat Protoc. 2020 Aug;15(8):2645-2667.Read more>>
  • Fei Wen. et al. TLR7/8 agonist (R848) inhibit bovine X sperm motility via PI3K/GSK3/ and PI3K/NFB pathways. INT J BIOL MACROMOL. 2023 Mar;232:123485Read more>>
  • Ling Shuai. et al. Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice. AM J REPROD IMMUNOL. 2023 FebRead more>>
  • Kohtaro Fukuyama. et al. Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium. FRONT IMMUNOL. 2023; 14: 1117102Read more>>
VALIDATION IMAGES

lane 1: Mouse brain; Lane 2: Mouse spleen lysates probed with Rabbit Anti-TLR7 Polyclonal Antibody (bs-6601R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C


Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human 293T cell lysates probed with TLR7 Polyclonal Antibody, Unconjugated (bs-6601R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.