bs-6624R [Primary Antibody]
IHH Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IHH

Immunogen Range: 161-260/411


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3549

Source: KLH conjugated synthetic peptide derived from human IHH

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ihh is an intercellular signalling peptide essential for a variety of patterning events during development. It binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Ihh induces the expression of parathyroid hormone-related protein (PTHRP) and is implicated in endochondral ossification, possibly regulating the balance between growth and ossification of the developing bones.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 45


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xijuan Liu et al. Chondrocyte suppression is mediated by miR\129\5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip. J Orthop Res. 2020 Dec;38(12):2559-2572.Read more>>
  • Xianda Cheet al. MicroRNA?1 regulates the development of osteoarthritis in a Col2a1?Cre?ERT2/GFPfl/fl?RFP?miR?1?Mousemodel of osteoarthritis through the downregulation of Indian hedgehog expression. Int J Mol Med. 2020 Jul;46(1):360-370.Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IHH Polyclonal Antibody, Unconjugated (bs-6624R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.