bs-6640R [Primary Antibody]
Vinculin Polyclonal Antibody
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]

Host: Rabbit

Target Protein: Vinculin

Immunogen Range: 1001-1134/1134

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7414

Swiss Prot: P18206

Source: KLH conjugated synthetic peptide derived from human Vinculin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.


Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 125

Cross Reactive Species: Human

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

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Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vinculin Polyclonal Antibody, Unconjugated (bs-6640R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.