bs-6640R [Primary Antibody]
Vinculin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Vinculin

Immunogen Range: 1001-1134/1134


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7414

Swiss Prot: P18206

Source: KLH conjugated synthetic peptide derived from human Vinculin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 125


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Wei, et al. "Tanshinone II a protects against liopolysaccharides-induced endothelial cell injury via Rho/Rho kinase pathway."Chinese Journal of Integrative Medicine 20 (2014): 216-223.Read more>>
  • Jiang, Yue-Hua, et al. "Calycosin-7-O-β-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells." BMC Complementary and Alternative Medicine 15.1 (2015): 315.Read more>>
  • Jiang, Y. H., et al. "Aqueous extracts of Tribulus terrestris protects against oxidized low-density lipoprotein-induced endothelial dysfunction." Chinese Journal of Integrative Medicine (2015).Read more>>
  • He, Jing, et al. "The essential role of inorganic substrate in the migration and osteoblastic differentiation of mesenchymal stem cells." Journal of the Mechanical Behavior of Biomedical Materials (2016).Read more>>
  • Dhawan U, Pan HA, Lee CH, Chu YH, Huang GS, Lin YR, et al. (2016) Spatial Control of Cell-Nanosurface Interactions by Tantalum Oxide Nanodots for Improved Implant Geometry. PLoS ONE 11(6): e0158425.Read more>>
  • Dhawan, Udesh, et al. "The Spatiotemporal Control of Osteoblast Cell Growth, Behavior, and Function Dictated by Nanostructured Stainless Steel Artificial Microenvironments." Nanoscale Research Letters 12.1 (2017): 86.Read more>>
  • Yunes-Medina, Laura, et al. "Depletion of transglutaminase 2 in neurons alters expression of extracellular matrix and signal transduction genes and compromises cell viability." Molecular and Cellular Neuroscience (2017).Read more>>
  • Haoxiang Chen. et al. 3D printed in vitro tumor tissue model of colorectal cancer. 2020 Oct 26Read more>>
  • Ruoyu Mu. et al. A Bridge-Building Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization. 2021 Sep 02Read more>>
  • Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022Read more>>
  • Xiaoting Lu. et al. Porous hydrogel constructs based on methacrylated gelatin/polyethylene oxide for corneal stromal regeneration. MATER TODAY COMMUN. 2022 Aug;32:104071Read more>>
  • Haibo Xiang. et al. Cryogenic 3D printing of bifunctional silicate nanoclay incorporated scaffolds for promoted angiogenesis and bone regeneration. MATER DESIGN. 2022 Oct;:11122Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vinculin Polyclonal Antibody, Unconjugated (bs-6640R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.