Host: Rabbit
Target Protein: Cytochrome P450 17A1
Immunogen Range: 24-65/508
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1586
Swiss Prot: P05093
Source: KLH conjugated synthetic peptide derived from human Cytochrome P450 17A1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
Predicted Molecular Weight: 57
Cross Reactive Species:
Mouse
Rat
Others
Predicted Cross Reactive Species:
Human
Cow
Sheep
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Wilsher, Sandra, et al. "Ovarian and placental morphology and endocrine function in the pregnant giraffe (Giraffa camelopardalis)." Reproduction (2013).Read more>>
- Han, Wentao, et al. "Seasonal Expression of P450c17 and 5α-reductase-2 in the Scented Gland of Male Muskrats (Ondatra zibethicus)." General and Comparative Endocrinology (2017).Read more>>
- Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
- Lu-ming Wu. et al. Dulaglutide, a long-acting GLP-1 receptor agonist, can improve hyperandrogenemia and ovarian function in DHEA-induced PCOS rats. Peptides. 2021 Nov;145:170624Read more>>
Mouse Testis lysates probed with Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1:rat brain lysates probed with Rabbit Anti-Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat testis）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 17A1) Polyclonal Antibody, Unconjugated (bs-6695R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates probed with Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6695R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.