Host: Rabbit
Target Protein: PP2A beta
Immunogen Range: 21-120/309
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5516
Swiss Prot: P62714
Source: KLH conjugated synthetic peptide derived from human PP2A beta/PPP2CB
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 36
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Cow
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded human colon caricinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with PP2A beta Polyclonal Antibody (bs-6706R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
U87mg cell lysates probed with PP2A beta Polyclonal Antibody, Unconjugated (bs-6706R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PP2A alpha + beta) Polyclonal Antibody, Unconjugated (bs-6706R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PP2A alpha + beta) Polyclonal Antibody, Unconjugated (bs-6706R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Rat Heart tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HepG2 cell lysates probed with PP2A alpha + beta Polyclonal Antibody, Unconjugated (bs-6706R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.