Host: Rabbit
Target Protein: GANAB
Specificity: This antibody may interact with GANC
Immunogen Range: 428-540/944
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 23193
Swiss Prot: Q14697
Source: KLH conjugated synthetic peptide derived from human GANAB/alpha Glucosidase II
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 104
Cross Reactive Species: Rat
Predicted Cross Reactive Species:
Human
Mouse
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded rat brain labeled with Anti P45017A1/Cytochrome P450 17A1 Polyclonal Antibody, Unconjugated (bs-6720R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti GANAB Polyclonal Antibody, Unconjugated (bs-6720R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 100kD. Observed band size: 100kD
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GANAB Polyclonal Antibody, Unconjugated (bs-6720R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GANAB Polyclonal Antibody, Unconjugated (bs-6720R) at 1:200 overnight at 4°C, DAB staining.