Host: Rabbit
Target Protein: Catalase
Immunogen Range: 251-350/527
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 847
Swiss Prot: P04040
Source: KLH conjugated synthetic peptide derived from human Catalase
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 60
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Horse
For research use only. Not intended for diagnostic or therapeutic use.
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Formalin-fixed and paraffin embedded rat kidney labeled with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 rat brain lysates probed with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 58kD. Observed band size: 58kD
Mouse liver lysates probed with Rabbit Anti-Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Catalase Polyclonal Antibody (bs-6874R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Placenta tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human HepG2 cell lysates probed with CAT Polyclonal Antibody, Unconjugated (bs-6874) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.