bs-6874R [Primary Antibody]
CAT Polyclonal Antibody
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Host: Rabbit

Target Protein: CAT

Immunogen Range: 251-350/527


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 847

Swiss Prot: P04040

Source: KLH conjugated synthetic peptide derived from human Catalase

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

Size: 100µl

Concentration: Lot Dependent

Applications: WB
ELISA
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
IHC-P
FCM

Predicted Molecular Weight: 60


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yun, Yang, et al. "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." Environmental Science & Technology (2017).Read more>>
  • Wang PW et al. Red Raspberry Extract Protects the Skin against UVB-Induced Damage with Antioxidative and Anti-inflammatory Properties. Oxid Med Cell Longev. 2019 Jan 6;2019:9529676. Read more>>
  • Liu R et al. Effect of Dietary Methionine Deficiency Followed by a Re-Feeding Phase on the Hepatic Antioxidant Activities of LambsAnimals (Basel).2020 Dec 23;11(1):7.Read more>>
  • Zhang Y et al. Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat modelPhytother Res.2020 Dec 10.Read more>>
  • Minjeong Kim. et al. Ibuprofen Increases the Hepatotoxicity of Ethanol through Potentiating Oxidative Stress. Biomol Ther. 2021 Mar 1; 29(2): 205C21Read more>>
  • Sheng Zhang. et al. Proteomics analysis in the kidney of mice following oral feeding Realgar. J Ethnopharmacol. 2021 Jul;275:114118Read more>>
  • Liu, Houli. et al. Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy. Nano Res. 2022 Feb;:1-1Read more>>
  • Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635Read more>>
  • Dr. Evan Paul Tracy. et al. Stromal Vascular Fraction Restores Vasodilatory Function by Reducing Oxidative Stress in Aging-Induced Coronary Microvascular Disease. ANTIOXID REDOX SIGN. 2022 Aug 11Read more>>
  • Shuang Wang. et al. Dioscin Protects against Cisplatin-Induced Acute Kidney Injury by Reducing Ferroptosis and Apoptosis through Activating Nrf2/HO-1 Signaling. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2443Read more>>
  • Yu Xia. et al. Astilbin targeted Sirt1 to inhibit acetylation of Nrf2 to alleviate grass carp hepatocyte apoptosis caused by PCB126-induced mitochondrial kinetic and metabolism dysfunctions. FISH SHELLFISH IMMUN. 2023 Oct;141:109Read more>>
  • Hong Zhao. et al. The anti-hyperplasia effect of polysaccharide from Prunella vulgaris L. on mammary gland hyperplasia in rats through regulation of the AKT-FOXO3a signaling pathway and intestinal flora. J SCI FOOD AGR. 2024 JuRead more>>
  • Shuang Wang. et al. Dioscin exerts nephroprotective effects by attenuating oxidative stress and necroptosis-induced inflammation. INT IMMUNOPHARMACOL. 2024 Oct;140:112885Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat kidney labeled with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) followed by conjugation to the secondary antibody and DAB staining


L1 rat kidney lysates L2 rat brain lysates probed with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 58kD. Observed band size: 58kD


Mouse liver lysates probed with Rabbit Anti-Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.


HL-60 cells were fixed with 4% PFA for 10min at room temperature,20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Catalase Polyclonal Antibody (bs-6874R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Placenta tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human HepG2 cell lysates probed with CAT Polyclonal Antibody, Unconjugated (bs-6874) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.