bs-6934R [Primary Antibody]
CD81 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD81

Immunogen Range: 101-210/236


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 975

Swiss Prot: P60033

Source: KLH conjugated synthetic peptide derived from human TAPA1/CD81

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 26


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fan Y et al. High-sensitive and multiplex biosensing assay of NSCLC-derived exosomes via different recognition sites based on SPRi array. Biosens Bioelectron. 2020 Apr 15;154:112066.Read more>>
  • Fan Y et al. High-sensitive and multiplex biosensing assay of NSCLC-derived exosomes via different recognition sites based on SPRi array. Biosens Bioelectron. 2020 Apr 15;154:112066.Read more>>
  • Jik-Han Jung. et al. Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma. Sci Rep-Uk. 2021; 11: 217Read more>>
  • Samanta Mecocci. et al. Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile. Nutrients. 2020 Oct;12(10):2908Read more>>
  • Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692Read more>>
  • Xiaoyi Gao. et al. Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. Acs Sensors. 2021;6(10):3611C362Read more>>
  • Hyesun Jeong. et al. GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer. Cancers. 2021 Jan;13(21):5482Read more>>
  • Shen, Xintong. et al. Evaluation of EpCAM-specific exosomal lncRNAs as potential diagnostic biomarkers for lung cancer using droplet digital PCR. 2021 Oct 14Read more>>
  • Samanta Mecocci. et al. Transcriptomic Characterization of Cow, Donkey and Goat Milk Extracellular Vesicles Reveals Their Anti-Inflammatory and Immunomodulatory Potential. Int J Mol Sci. 2021 Jan;22(23):12759Read more>>
  • Li, Kanglu. et al. Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages. J Nanobiotechnol. 2022 Dec;20(1):1-2Read more>>
  • Yinfeng Chen. et al. Serum exosomal hsa_circ_0069313 has a potential to diagnose more aggressive non-small cell lung cancer. Clin Biochem. 2022 JaRead more>>
  • Yinfeng Chen. et al. PLA2G10 incorporated in exosomes could be diagnostic and prognostic biomarker for non-small cell lung cancer. Clin Chim Acta. 2022 FebRead more>>
  • Jingsong Chen. et al. Exploring the Temporal Correlation of Sarcopenia with Bone Mineral Density and the Effects of Osteoblast-Derived Exosomes on Myoblasts through an Oxidative Stress-Related Gene.. OXID MED CELL LONGEV. 2022 Sep;2022:9774570-977457Read more>>
  • Fengchun Liao. et al. ECFC-derived exosomal THBS1 mediates angiogenesis and osteogenesis in distraction osteogenesis via the PI3K/AKT/ERK pathway. J ORTHOP TRANSL. 2022 Nov;37:12Read more>>
  • Yutong Wu. et al. Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. CELL SIGNAL. 2022 Dec;:110549Read more>>
  • Samanta Mecocci. et al. Antimicrobial and Immunomodulatory Potential of Cow Colostrum Extracellular Vesicles (ColosEVs) in an Intestinal In Vitro Model. BIOMEDICINES. 2022 Dec;10(12):3264Read more>>
  • Jinglei Wang. et al. Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro. J CELL PHYSIOL. 2023 ApRead more>>
  • Panpan Liu. et al. Endothelial progenitor cell-derived exosomes inhibit pulmonary artery smooth muscle cell in vitro proliferation and resistance to apoptosis by modulating the Mitofusin-2 and Ras-Raf-ERK1/2 signaling pathway. EUR J PHARMACOL. 2023 Apr;:175725Read more>>
  • Franzoni Giulia. et al. Goat milk extracellular vesicles: immuno-modulation effects on porcine monocyte-derived macrophages in vitro. FRONT IMMUNOL. 2023 Jun;14Read more>>
  • Chengtao Lou. et al. The mtDNA fragments within exosomes might be novel diagnostic biomarkers of non-small cell lung cancer. PATHOL RES PRACT. 2023 Sep;249:154718Read more>>
  • Dai Lei. et al. The diagnostic potential of two exosome-derived circRNAs for papillary thyroid cancer. INT J CLIN ONCOL. 2023 Aug;:1-14Read more>>
  • in Ma. et al. Down-regulation of COL1A1 inhibits tumor-associated fibroblast activation and mediates matrix remodeling in the tumor microenvironment of breast cancer. OPEN LIFE SCI. 2023 Jan;18(1)Read more>>
  • Xiaofeng Chen. et al. Exosomal U2AF2 derived from human bone marrow mesenchymal stem cells attenuates the intervertebral disc degeneration through circ_0036763/miR-583/ACAN axis. REGEN THER. 2024 Mar;25:344Read more>>
VALIDATION IMAGES

Mouse spleen lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Jurkat cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1:U937 cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lung lysates; Lane 4: Mouse Thymus lysates; Lane 5: Mouse RAW264.7 cell lysates; Lane 6: Human THP-1 cell lysates; Lane 7: Human Raji cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.