bs-6961R [Primary Antibody]
ASMT Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ASMT

Immunogen Range: 241-345/345


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 438

Swiss Prot: P46597

Source: KLH conjugated synthetic peptide derived from human Acetylserotonin O-methyltransferase (ASMT)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene. Isoform 1 catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Isoform 2 and isoform 3 lack enzyme activity

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 38


Cross Reactive Species: Rat
Sheep
Others

Predicted Cross Reactive Species: Human
Mouse
Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Herman, A. P., et al. "Interleukin-1β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study." BioMed Research International (2015).Read more>>
  • Herman, A. P., et al. "Central Interleukin-1β Suppresses the Nocturnal Secretion of Melatonin." Mediators of Inflammation 2016 (2016).Read more>>
  • Herman et al. Endotoxin-induced inflammation disturbs melatonin secretion in ewe. (2017) Asian-Australas.J.Anim.Sci. 30:1784-1795Read more>>
  • Zhang, WL.. et al. Expression of arylalkylamine n-acetyltransferase (AANAT) and acetylserotonin o-methyltransferase (ASMT) in the corpus luteum of pregnant sows and synthesis of melatonin in luteal cells. 2021 Nov 24Read more>>
  • Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 AugRead more>>
  • Huawei Wei. et al. Melatonin through blockade of Hif-1 signaling mediates the anti-fibrosis under hypoxia in canine Sertoli cells. REPROD BIOL. 2022 Dec;22:100677Read more>>
  • C. Sosa. et al. The melatonin system is expressed in the ovine uterus: effect of the day of the oestrous cycle and undernutrition. REPROD FERT DEVELOP. 2023 JuRead more>>
  • Jinho Kim. et al. Biosynthesis of neuroprotective melatonin is dysregulated in Huntington's disease. J PINEAL RES. 2023 Sep;:e12909Read more>>
VALIDATION IMAGES

Rat brain lysate probed with Rabbit Anti-ASMTY Polyclonal Antibody, Unconjugated (bs-6961R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.