DATASHEET
Host:
Rabbit
Target Protein:
SEMA4D
Immunogen Range:
75-170/862
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
10507
Swiss Prot:
Q92854
Source:
KLH conjugated synthetic peptide derived from human SEMA4D
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.
PRODUCT SPECIFIC PUBLICATIONS
- Sun Shin. et al. Leprosy-specific subsets of macrophages and Schwann cells identified by single-cell RNA-sequencing. PATHOL RES PRACT. 2023 Oct;250:154821Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human spleen tissue labeled with Anti-SEMA4D/CD100 Polyclonal Antibody, Unconjugated (bs-6965R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SEMA4D) Polyclonal Antibody, Unconjugated (bs-6965R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.