bs-6970R [Primary Antibody]
FOXN1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FOXN1

Immunogen Range: 321-420/648


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8456

Swiss Prot: O15353

Source: KLH conjugated synthetic peptide derived from human FOXN1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 69


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Volpi, Stefano, et al. "EXTL3 mutations cause skeletal dysplasia, immune deficiency, and developmental delay." Journal of Experimental Medicine (2017): jem-20161525.Read more>>
  • Kolb AD et al. Osteoblasts are “educated” by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment.Breast Cancer Res. 2019 Feb 27;21(1):31. Read more>>
  • Guo L et al. Gallic acid attenuates thymic involution in the D-galactose induced accelerated aging mice. Immunobiology. 2019.Read more>>
  • Yuyan Wang. et al. ET-1 regulates the human umbilical vein endothelial cell cycle by adjusting the ER/FOXN1 signaling pathway. Ann Transl Med. 2020 Nov; 8(22): 1499Read more>>
  • Ting\ting Wei. et al. Dosage effects of resveratrol on thymus involution in D\galactose\treated mice. 2021 Mar 28Read more>>
  • Shu-ping Yang. et al. Metformin ameliorates thymus degeneration of mice by regulating mitochondrial function. INT IMMUNOPHARMACOL. Int Immunopharmacol. 2022 Jul;108:108744Read more>>
  • Zeleniak, Ann. et al. De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids. NAT METHODS. 2022 Sep;:1-14Read more>>
VALIDATION IMAGES

Lane 1:rat thymus lysates probed with Rabbit Anti-FOXN1 Polyclonal Antibody, Unconjugated (bs-6970R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.