bs-6973R [Primary Antibody]
Mkl1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Mkl1

Immunogen Range: 201-300/931


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 57591

Swiss Prot: Q969V6

Source: KLH conjugated synthetic peptide derived from human Mkl1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 45, 90


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lin Niu. et al. Magnolol alleviates pulmonary fibrosis inchronic obstructive pulmonary disease by targeting transient receptor potential vanilloid 4-ankyrin repeat domain. PHYTOTHER RES. 2023 JuRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti Mkl1/MRTF-A Polyclonal Antibody, Unconjugated (bs-6973R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Protein: line1,mouse brain;line2,mouse lung;<br>\nPrimary: Anti-Mkl1 (bs-6973R) at 1:300; <br>\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000; <br>\nECL excitated the fluorescence; <br>\nPredicted band size :45/90 kD<br>\nObserved band size : 45/90 kD<br>\n