bs-6981R [Primary Antibody]
CLCN3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CLCN3

Immunogen Range: 81-180/818


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1182

Swiss Prot: P51790

Source: KLH conjugated synthetic peptide derived from human CLCN3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 91


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • lair HC et al. Support of Bone Mineral Deposition by Regulation of pH.Am J Physiol Cell Physiol. 2018 Oct 1;315(4):C587-C597. Read more>>
  • lair HC et al. Support of Bone Mineral Deposition by Regulation of pH.Am J Physiol Cell Physiol. 2018 Oct 1;315(4):C587-C597. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CLCN3 Polyclonal Antibody, Unconjugated (bs-6981R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CLCN3 Polyclonal Antibody, Unconjugated (bs-6981R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.