bs-6982R
[Primary Antibody]
Neutrophil Elastase Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Neutrophil Elastase
Immunogen Range: 101-200/267
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1991
Swiss Prot: P08246
Source: KLH conjugated synthetic peptide derived from human Neutrophil Elastase
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 26
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jeffery, Unity, et al. "Dogs Cast NETs too: Canine neutrophil extracellular traps in health and immune-mediated hemolytic anemia." Veterinary Immunology and Immunopathology (2015).Read more>>
- Arecco, N., et al. "Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation."Scientific Reports 6 (2016): 24708.Read more>>
- Maekawa, Toshihiro, et al. "Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model [Errata]." Medical mycology journal 57.4 (2016): E81-E92.Read more>>
- Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775.Read more>>
- Krishnan, Subramanian, et al. "Serotype O18 avian pathogenic and neonatal meningitis Escherichia coli strains employ similar pathogenic strategies for the onset of meningitis." Virulence 6.8 (2015): 777-786.Read more>>
- Peng et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. (2018) J.Biomed.Sci. 25:24Read more>>
- Arecco et al. Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation. (2016) Sci.Rep. 6:24708Read more>>
- Ye, Y. et al. CXCR1/CXCR2 antagonist G31P inhibits nephritis in a mouse model of uric acid nephropathy.(2018) BIOMED PHARMACOTHERRead more>>
- Störmann P et al. Contributing factors in the development of acute lung injury in a murine double hit model. Eur J Trauma Emerg Surg. 2019 Apr 1.Read more>>
- Wang H et al. The effect of exposure time and concentration of airborne PM2. 5 on lung injury in mice: A transcriptome analysis. Redox Biol. 2019 Jul 2;26:101264. Read more>>
- Gao J et al. Metformin protects against PM2. 5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2. Redox Biol. 2019 Oct 19;28:101345.Read more>>
- Vaibhav K et al. Neutrophil extracellular traps exacerbate neurological deficits after traumatic brain injury. Sci Adv.2020 May 29;6(22):eaax8847.Read more>>
- Sladjana Skopelja-Gardner. et al. Acute skin exposure to ultraviolet light triggers neutrophil-mediated kidney inflammation. P Natl Acad Sci Usa. 2021 Jan;118(3)Read more>>
- Andrew R. Sas. et al. A new neutrophil subset promotes CNS neuron survival and axon regeneration. Nat Immunol. 2020 Oct;21(12):1496-1505Read more>>
- Qin Tan. et al. Periodontitis pathogen Porphyromonas gingivalis promotes pancreatic tumorigenesis via neutrophil elastase from tumor-associated neutrophils. GUT MICROBES. 2022;14(1):2073785Read more>>
- Yongcan Wu. et al. Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury. ECOTOX ENVIRON SAFE. 2022 Oct;244:11406Read more>>
- Cao Yirui. et al. Formyl peptide receptor 2 activation by mitochondrial formyl peptides stimulates the neutrophil proinflammatory response via the ERK pathway and exacerbates ischemiaCreperfusion injury. CELL MOL BIOL LETT. 2023 Dec;28(1):1-24Read more>>
- Li Xiangdong. et al. Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3. J TRANSL MED. 2023 Dec;21(1):1-19Read more>>
- Hannah M. Lunnemann. et al. Cecum axis (CecAx) preservation reveals physiological and pathological gradients in mouse gastrointestinal epithelium. GUT MICROBES. 2023;15(1):Article: 2185029Read more>>
- Luchen Sun. et al. A novel mesenchymal stem cell-based regimen for acute myeloid leukemia differentiation therapy. ACTA PHARM SIN B. 2023 MayRead more>>
- Fanshuai Meng. et al. Impact of age on liver damage, inflammation, and molecular signaling pathways in response to femoral fracture and hemorrhage. FRONT IMMUNOL. 2023; 14: 1239145Read more>>
VALIDATION IMAGES
A549 cells probed with \tNeutrophil Elastase Polyclonal Antibody, Unconjugated (bs-6982R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse spinal cord lysates probed with Neutrophil Elastase Polyclonal Antibody, unconjugated (bs-6982R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: HL-60 cell lysates probed with Neutrophil Elastase Polyclonal Antibody, Unconjugated (bs-7537R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Bone tissue lysates; Lane 2: Rat Bone tissue lysates probed with Neutrophil Elastase Polyclonal Antibody, Unconjugated (bs-6982R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Neutrophil Elastase Polyclonal Antibody(bs-6982R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).