Host: Rabbit
Target Protein: Wilms Tumor Protein
Immunogen Range: 301-400/449
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7490
Swiss Prot: P19544
Source: KLH conjugated synthetic peptide derived from human Wilms Tumor Protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Wang X et al. Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway. Theriogenology. 2019 Jul 8;138:84-93. Read more>>
- Wang X et al. Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells. Reprod Fertil Dev. 2020 Feb 6. Read more>>
- Wang X et al. Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells. Reprod Fertil Dev. 2020 Feb 6. Read more>>
- Rui Feng. et al. The ameliorative effect of melatonin on LPS-induced Sertoli cells inflammatory and tight junctions damage via suppression of the TLR4/MyD88/NF-B signaling pathway in newborn calf. Theriogenology. 2022 Feb;179:103Read more>>
- Wenjun Chen. et al. Salvianolate Ameliorates Renal Damage Induced by C-BSA in Membranous Nephropathy Rats Through Inhibiting Hypercoagulable State and Alleviating Podocyte Injury. PHARMACOGN MAG. ;()Read more>>
- Eun-Jeong Kwon. et al. Development of Drug Efficacy Testing Platform for Glomerulonephritis. MICROMACHINES-BASEL. 2024 Mar;15(3):317Read more>>
L1 human colon carcinoma lysates L2 rat brain lysates probed with Anti- WT-1/Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 55kD. Observed band size: 55kD
Formalin-fixed and human kidney tissue labeled with Anti-WT-1/Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Ovary tissue lysates; Lane 3: Mouse Liver tissue lysates; Lane 4: Human HL-60 cell lysates probed with WT1 Polyclonal Antibody, Unconjugated (bs-6983R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.