bs-6988R [Primary Antibody]
RUBISCO Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RUBISCO

Immunogen Range: 151-250/479


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 844754

Source: KLH conjugated synthetic peptide derived from Arabidopsis thaliana RUBISCO

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.Sequence similarities: Belongs to the RuBisCO large chain family. Type I subfamily.Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Plant
Others
(Arabidopsis thaliana,)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Tai-Jie, et al. "A magic red coat on the surface of young leaves: anthocyanins distributed in trichome layer protect Castanopsis fissa leaves from photoinhibition." Tree Physiology (2016).Read more>>
  • Cheng S et al. Differential accumulation of specialized metabolite L-theanine in green and albino-induced yellow tea (Camellia sinensis) leaves.(2019) Food Chemistry. 276:93-100Read more>>
  • Bohley K et al. C4-like photosynthesis and the effects of leaf senescence on C4-like physiology in Sesuvium sesuvioides (Aizoaceae). J Exp Bot. 2019 Mar 11;70(5):1553-1565. Read more>>
  • Zhang Q et al. The Changing Distribution of Anthocyanin in Mikania micrantha Leaves as an Adaption to Low-Temperature Environments. Plants (Basel). 2019 Oct 27;8(11). pii: E456.Read more>>
VALIDATION IMAGES

Lane1,4,7: Calabas lysates; Lane 2,5,8: Lotus leaf lysates; Lane 3,6,9: Syzgium caryophyllm lysates probed with Rabbit Anti-RUBISCO Polyclonal Antibody, Unconjugated (bs-6988R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.