bs-70017R

DATASHEET

Host: Rabbit

Target Protein: Aquaporin 2 (Ser261)

Specificity: Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser261

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25386

Swiss Prot: P34080

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 of rat aquarporin 2, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007)

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 29/37

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Bovine
Chicken
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.