bs-70035R

DATASHEET

Host: Rabbit

Target Protein: cdc2 (Tyr15)

Specificity: Specific for endogenous levels of the ~34 kDa cdc2 protein phosphorylated at Tyr15. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Tyr15

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 54237

Swiss Prot: P39951

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 of rat cdc2, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr-15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 34

Cross Reactive Species: Human
Mouse
Xenopus

Predicted Cross Reactive Species: Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.