bs-70041R

DATASHEET

Host: Rabbit

Target Protein: Che-1 (AATF) (Ser477)

Specificity: Specific for endogenous levels of the ~66 kDa Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Modification Site: Ser477

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 26574

Swiss Prot: Q9NY61

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)

Predicted Molecular Weight: 66

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Bovine
Sheep
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.