bs-70046R

DATASHEET

Host: Rabbit

Target Protein: Collagen 1, alpha 1 telopeptide

Specificity: Specific for endogenous levels of the ~140 kDa collagen I alpha-1 telopeptide portion of the collagen I alpha-1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0) / 0.1% Tween-20).

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1277

Swiss Prot: P02452

Source: Synthetic peptide corresponding to amino acid residues specific to the C-terminal region of human collagen I alpha 1 telopeptide, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification

Storage Buffer: PBS

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliq

Background:

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the most abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the first 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).

Size: 100ul

Concentration: Lot dependent

Predicted Molecular Weight: 140

Cross Reactive Species: Human
Mouse
Rat
Sheep

Predicted Cross Reactive Species: Dog
Bovine
Horse
Chicken
Cat
Rabbit
Guinea Pig
Goat
Hamster
Xenopus
Non-Human Primate
Vole
Finch

For research use only. Not intended for diagnostic or therapeutic use.