bs-70073R

DATASHEET

Host: Rabbit

Target Protein: DsbA-L

Specificity: Specific for endogenous levels of the ~25 kDa DsbA-L protein. It is suggested that you add 1% BSA to the antibody dilution buffer.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q9DCM2

Source: Full length recombinant mouse DsbA-L protein.

Purification: whole antiserum

Storage Buffer: whole antiserum in 40% glycerol

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy

Background:

Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution (Gao F et al., 2009). Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis (Liu et al., 2008).

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC

Predicted Molecular Weight: 25

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.