bs-70127R

DATASHEET

Host: Rabbit

Target Protein: Glycine Receptor

Specificity: Specific for endogenous levels of the ~48 kDa α1- and α2-subunits of the glycine receptor. Immunolabeling is blocked by preadsorption of the antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25674

Swiss Prot: P07727

Source: Synthetic peptide corresponding to amino acid residues from the N-terminal region of the α1 subunit of rat glycine, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: Lyophilized from 5 mM ammonium biocarbonate.

Storage: The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that th

Background:

Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).