bs-70182R

DATASHEET

Host: Rabbit

Target Protein: NMDA NR1 Subunit, Splice Variant C2'

Specificity: Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor containing the C2’ splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2’ insert.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24408

Swiss Prot: P35439

Source: Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C2' splice variant insert of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: Lyophilized

Storage: Reconstitute in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be

Background:

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). Increased membrane surface expression of the NMDAR, NR1-Subunit has been associated with synaptic plasticity (Grosshans et al., 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.