bs-70225R

DATASHEET

Host: Rabbit

Target Protein: Polo-Like Kinase Kinase (Ser482,486,490)

Specificity: Specific for endogenous levels of the ~120k PLKK protein phosphorylated at Ser482,486,490.

Modification Site: Ser482,486,490

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 380481

Swiss Prot: B7ZR30

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus polo-like kinase kinase (PLKK), conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser-482, Ser-486 and Ser-490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser-482,486,490 in PLKK.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 120

Cross Reactive Species: Rat
Xenopus

Predicted Cross Reactive Species: Human
Mouse
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.