bs-70230R

DATASHEET

Host: Rabbit

Target Protein: PRAS40 (Thr356)

Specificity: Specific for endogenous levels of the ~60 kDa PRAS40 protein phosphorylated at Thr356. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Thr356

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q7JXA2

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 of Drosophila PRAS40, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt- and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al., 2012). Phosphorylation of PRAS40 by Akt and association with 14-3-3 has also been indicated to be crucial for insulin to stimulate mTOR. (Vander Haar et al., 2007). The primary function of PRAS40 in vivo in Drosophila has been shown to regulate TORC1 activity, and not to act as a downstream target and effector of TORC1 (Pallares et al., 2012).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 60

Cross Reactive Species: Drosophila

For research use only. Not intended for diagnostic or therapeutic use.