bs-7044R [Primary Antibody]
EphB1+EphB2 (Tyr594) Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EphB1+EphB2 Tyr594

Modification Site: Tyr594

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2047

Source: KLH conjugated synthetic phosphopeptide derived from human EphB1+EphB2 around the phosphorylation site of Tyr594

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Bovine

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Mouse NIH/3T3 cell lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Rat Cerebellum tissue lysates; Lane 7: Rat Placenta tissue lysates; Lane 8: Human U251 cell lysates; Lane 9: Human U87MG cell lysates; Lane 10: Human MCF-7 cell lysates; Lane 11: Human U-2 OS cell lysates probed with EphB1+EphB2 Polyclonal Antibody, Unconjugated (bs-7044R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.