Host: Rabbit
Target Protein: MYL9 Ser20
Modification Site: Ser20
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10398
Swiss Prot: P24844
Source: KLH conjugated synthetic phosphopeptide derived from human Myosin Regulatory Light polypeptide 9 around the phosphorylation site of Ser20
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 20
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Li, Wei, et al. "Tanshinone II a protects against liopolysaccharides-induced endothelial cell injury via Rho/Rho kinase pathway." Chinese Journal of Integrative Medicine 20 (2014): 216-223.Read more>>
- Hoj, Jacob P., et al. "Cellular contractility changes are sufficient to drive epithelial scattering." Experimental Cell Research (2014).Read more>>
- Zhuang S et al. Rhein protects against barrier disruption and inhibits inflammation in intestinal epithelial cells. Int Immunopharmacol. 2019 Jun;71:321-327.Read more>>
- Su H et al. cis 9, trans 11, but not trans 10, cis 12 CLA isomer, impairs intestinal epithelial barrier function in IPEC-J2 cells and mice through activation of GPR120-[Ca2+]i and the MLCK signaling pathway. Food Funct. 2020 Apr 30;11(4):3657-3667. Read more>>
- Jinwen Yuan. et al. Reparative Effects of Ethanol-Induced Intestinal Barrier Injury by Flavonoid Luteolin via MAPK/NF-B/MLCK and Nrf2 Signaling Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
- Luqing Song. et al. Chlorogenic acid improves the intestinal barrier by relieving endoplasmic reticulum stress and inhibiting ROCK/MLCK signaling pathways.. Food Funct. 2022 FebRead more>>
- Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858Read more>>
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-phospho-MLC(Ser20)/Myosin light chain(Ser20) Polyclonal Antibody, Unconjugated (bs-7052R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-phospho-MLC(Ser20)/Myosin light chain(Ser20) Polyclonal Antibody, Unconjugated (bs-7052R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human kidney labeled with Anti-phospho-MLC(Ser20)/Myosin light chain(Ser20) Polyclonal Antibody, Unconjugated(bs-7052R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.