bs-7056R [Primary Antibody]
FTO Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FTO

Immunogen Range: 165-265/505


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 79068

Swiss Prot: Q9C0B1

Source: KLH conjugated synthetic peptide derived from human Fat mass and obesity-associated protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tengjiao Qu. et al. Changes and relationship of N6-methyladenosine modification and long non-coding RNAs in oxidative damage induced by cadmium in pancreatic -cells. Toxicol Lett. 2021 Jun;343:56Read more>>
  • Jun Zhang. et al. To Develop and Validate the Combination of RNA Methylation Regulators for the Prognosis of Patients with Gastric Cancer. Oncotargets Ther. 2020; 13: 10785C10795Read more>>
  • Cui Yuanbo. et al. RNA m6A demethylase FTO-mediated epigenetic up-regulation of LINC00022 promotes tumorigenesis in esophageal squamous cell carcinoma. J Exp Clin Canc Res. 2021 Dec;40(1):1-2Read more>>
  • Leqiang Gao. et al. Fat mass and obesity-associated gene (FTO) hypermethylation induced by decabromodiphenyl ethane causing cardiac dysfunction via glucolipid metabolism disorder. ECOTOX ENVIRON SAFE. 2022 Jun;237:113534Read more>>
  • Jiaojiao Yang. et al. FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein. ANIMALS. 2022 Jan;12(13):1644Read more>>
  • Wang, Xiaoying. et al. LncRNA FENDRR with m6A RNA methylation regulates hypoxia-induced pulmonary artery endothelial cell pyroptosis by mediating DRP1 DNA methylation. MOL MED. 2022 Dec;28(1):1-2Read more>>
  • Yuanchao Ling. et al. Bisphenol A exposure induces testicular oxidative damage via FTO/m6A/Nrf2 axis during postnatal development in mice. J APPL TOXICOL. 2022 NovRead more>>
  • Xuhua Sun. et al. Identification of clinical heterogeneity and construction of a novel subtype predictive model in patients with ankylosing spondylitis: An unsupervised machine learning study. INT IMMUNOPHARMACOL. 2023 Apr;117:109879Read more>>
  • Chunyan Zhang. et al. Homeobox-D 1 and FTO form a transcriptionalCepigenetic feedback loop to promote head and neck cancer proliferation. CELL BIOL INT. 2023 SepRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-FTO/Protein Fatso Polyclonal Antibody, Unconjugated (bs-7056R) at 1:200 followed by conjugation to the secondary antibody