Host: Rabbit
Target Protein: B-Raf (S446)[C-Raf (S338)/A-Raf (S299)
Specificity: This antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide. The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or A431 cells treated with Calyculin A.
Modification Site: Ser-446
Clonality: Polyclonal
Isotype: IgG
Swiss Prot: P15056
Source: Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf.
Purification: Antigen Affinity purification
Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Background:
Size: 100ul
Predicted Molecular Weight: 95/105
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.