bs-70648R

DATASHEET

Host: Rabbit

Target Protein: SCAI (N-terminal region)

Specificity: The antibody detects a significant 66 kDa* protein corresponding to the molecular mass of SCAI on SDS-PAGE immunoblots of mouse brain and human A431 cells. In brain, the antibody also detects 53 and 41 kDa bands.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q8C8N2

Source: SCAI synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of mouse SCAI. This peptide sequence is highly conserved in human, rat, mouse, and chicken SCAI.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Cellular processes such as proliferation, differentiation, and cell migration are directed through the highly regulated control of transcriptional cofactors. During cell migration, whole antiserum response factor (SRF) is a central transcription factor that interacts with MAL to induce gene expression in response to actin assembly changes caused by the RhoA-mDia1 pathway. SCAI (suppressor of cancer cell invasion) is a highly conserved protein that is thought to regulate invasive cell migration and act on the RhoA–mDia1 signal transduction pathway. SCAI localizes in the nucleus, where it is thought to bind and inhibit MAL, by forming a ternary complex with SRF. Suppression of SCAI expression has been shown to upregulate β1-integrin expression, an intergrin that is also upregulated in several human malignancies. In addition, SCAI is downregulated in various human tumors, and decreased levels of SCAI correlate with increased invasive cell migration. Thus, SCAI is a transcriptional cofactor that may be important for regulating gene expression downstream of mDia1 to direct changes in cell motility.