bs-7112R [Primary Antibody]
S1P1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: S1P1

Immunogen Range: 51-150/382

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1901

Swiss Prot: P21453

Source: KLH conjugated synthetic peptide derived from human S1P1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis (By similarity). Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiao et al. Fingolimod Suppresses a Cascade of Core Vicious Cycle in Dry Eye NOD Mouse Model: Involvement of Sphingosine-1-Phosphate Receptors in Infiltrating Leukocytes. (2017) Invest.Ophthalmol.Vis.Sci. 58:6123-6132Read more>>
  • Gu X et al. SPNS 2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway. Clin Exp Pharmacol Physiol. 2019 Jun 17. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-EDG1/CD363/S1P1 Polyclonal Antibody, Unconjugated (bs-7112R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Rat heart lysates probed with S1P1 Polyclonal Antibody, unconjugated (bs-7112R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Lane 1: HUVEC Cells; Probed with S1P1 Polyclonal Antibody (bs-7112R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.