bs-7128R

DATASHEET

Host: Rabbit

Target Protein: Perforin

Immunogen Range: 22-100/555

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5551

Source: KLH conjugated synthetic peptide derived from human Perforin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The major defense of the body against virus-infected and tumorigenic cells is cytotoxic T lymphocyte (CTL)-mediated cytotoxicity, which also plays a role in autoimmune diseases and transplant rejection. During CTL-mediated cytotoxicity, CTL granules containing perforin are exocytosed. Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells by forming transmembrane channels in target cell membranes. Perforin is primarily expressed in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, but has also been observed in an astrocyte population of the human brain. It has been shown that abrogation of perforin function by Ca2+-complexing agents leads to decreased levels of necrosis, demonstrating that both necrosis and apoptosis contribute to CTL-mediated cytotoxicity. Perforin activity has been shown to be induced by IL-2, IL-3, IL-4, IL-6 and to a lesser degree, TNF and IFN-_.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 59

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Horse

For research use only. Not intended for diagnostic or therapeutic use.