bs-7133R-A350

DATASHEET

Host: Rabbit

Target Protein: RRM2

Immunogen Range: 151-250/389

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6241

Source: KLH conjugated synthetic peptide derived from human RRM2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Ribonucleotide reductase is essential for the production and maintenance of the level of deoxyribonucleoside triphosphates (dNTPs) required for DNA synthesis. It is an enzymatic complex consisting of two nonidentical subunits, R1 and R2, which are inactive separately. R2, the smaller subunit, is localized to the cytoplasm. R2 is the limiting factor of the catalytic activity of the ribonucleotide reductase enzymatic complex. R2 expression is strictly correlated to the S-phase of the cell cycle, whereas R1 remains constant throughout all phases of the cell cycle. While R2 seems to be involved solely in the maintenance of dNTPs for DNA replication, a similar protein, p53R2, has been shown to be responsible for the production of dNTPs in response to DNA damage.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 45

Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.