bs-7158R [Primary Antibody]
Collagen I Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Collagen I

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1277

Source: KLH conjugated synthetic peptide derived from human Collagen alpha-1(I) chain

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang et al. Effect of Shenkang granules on the progression of chronic renal failure in 5/6 nephrectomized rats. (2015) Exp.Ther.Me. 9:2034-2042Read more>>
  • Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182.Read more>>
  • Du XS et al. Wogonin attenuates liver fibrosis via regulating hepatic stellate cell activation and apoptosis. Int Immunopharmacol. 2019 Aug 1;75:105671.Read more>>
  • Yang Ling. et al. Three-dimensional (3D) hydrogel serves as a platform to identify potential markers of chondrocyte dedifferentiation by combining RNA sequencing. Bioact Mater. 2021 Sep;6:2914Read more>>
  • Rui Zhang. et al. Allogeneic adipose-derived mesenchymal stem cells promote the expression of chondrocyte redifferentiation markers and retard the progression of knee osteoarthritis in rabbits. Am J Transl Res. 2021; 13(2): 632C645Read more>>
  • Xing Su. et al. Mushroom Inonotus sanghuang alleviates experimental pulmonary fibrosis: Implications for therapy of pulmonary fibrosis. Biomed Pharmacother. 2021 Jan;133:110919Read more>>
  • Han Li. et al. Low\intensity pulsed ultrasound promotes the formation of periodontal ligament stem cell sheets and ectopic periodontal tissue regeneration. 2020 Sep 3Read more>>
  • Yang Zhang. et al. Plumbagin Inhibits Proliferation, Migration, and Invasion of Retinal Pigment Epithelial Cells Induced by FGF-2. Tissue Cell. 2021 Oct;72:101547Read more>>
  • Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF?1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13Read more>>
  • Ao Wang. et al. MicroRNA-195-3p promotes hepatic stellate cell activation and liver fibrosis by suppressing PTEN expression. Toxicol Lett. 2022 Feb;355:88Read more>>
  • Qi-Lin Lu. et al. Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy. MOL MED REP. 2022 Sep;26(3):1-9Read more>>
  • Tingting Jiang. et al. Pathological Changes in Hepatic Sinusoidal Endothelial Cells in Schistosoma japonicum-Infected Mice. Tropical Medicine and Infectious Disease. 2023 Feb;8(2):124Read more>>
  • Yuan Gao. et al. Inhibition of hepatic natural killer cell function via the TIGIT receptor in schistosomiasis-induced liver fibrosis. PLOS PATHOG. 2023 Mar;19(3):e1011242Read more>>
  • Yue Du. et al. Decorin inhibits the formation of hard nodules after microwave ablation by inhibiting the TGF-1/SMAD and MAPK signaling pathways: in a Bama miniature pig model of mammary gland hyperplasia. INT J HYPERTHER. 2023;40(1):Article: 2188151Read more>>
  • Li Gao. et al. STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease. MOL THER. 2023 Aug 01Read more>>
  • Xiaoying Zhao. et al. A novel adhesive dual-sensitive hydrogel for sustained release of exosomes derived from M2 macrophages promotes repair of bone defects. MATER TODAY BIO. 2023 Nov;:10084Read more>>
  • Xiaofeng Chen. et al. Exosomal U2AF2 derived from human bone marrow mesenchymal stem cells attenuates the intervertebral disc degeneration through circ_0036763/miR-583/ACAN axis. REGEN THER. 2024 Mar;25:344Read more>>
VALIDATION IMAGES

Lane 1: Mouse Placenta tissue lysates; Lane 2: Human U-2OS cell lysates probed with Collagen I Polyclonal Antibody, Unconjugated (bs-7158R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.