bs-7166R [Primary Antibody]
MUC5AC Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MUC5AC

Immunogen Range: 601-700/5030


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4586

Swiss Prot: P98088

Source: KLH conjugated synthetic peptide derived from human MUC5AC

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 523


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Taki, K., et al. "GNASR201H and KrasG12D cooperate to promote murine pancreatic tumorigenesis recapitulating human intraductal papillary mucinous neoplasm." Oncogene 35.18 (2016): 2407-2412.Read more>>
  • Tomchaney, M.. et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease. Bmc Pulm Med. 2021 Dec;21(1):1-14Read more>>
  • Mohamed Elbadawy. et al. Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus. Int J Mol Sci. 2021 Jan;22(19):10763Read more>>
  • Sasagu Kimura. et al. Histological characteristics of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in asthmatic murine model during A(H1N1)pdm09 infection. PATHOL INT. 2022 SepRead more>>
  • Lu Liu. et al. Scutellarein alleviates chronic obstructive pulmonary disease through inhibition of ferroptosis by chelating iron and interacting with arachidonate 15-lipoxygenase. PHYTOTHER RES. 2023 JuRead more>>
  • Lihua Hu. et al. Fine particulate matter promotes airway inflammation and mucin production by activating endoplasmic reticulum stress and the IRE1/NOD1/NF?B pathway. INT J MOL MED. 2023 Oct;52(4):1-13Read more>>
  • Mohammed Imran Khan. et al. Method for Collecting Single Epithelial Cells from the Mouse Larynx. LARYNGOSCOPE. 2023 AugRead more>>
  • Dr. Yasuyuki Hayashi. et al. Comparative study of immunodeficient rat strains in engraftment of hiPSC-derived airway epithelia. Tissue Engineering Part A. 2023 Nov 11Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MUC5AC Polyclonal Antibody, Unconjugated (bs-7166R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining


Paraformaldehyde-fixed, paraffin embedded mouse stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MUC5AC Polyclonal Antibody, Unconjugated (bs-7166R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MUC5AC Polyclonal Antibody, Unconjugated (bs-7166R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MUC5AC Polyclonal Antibody, Unconjugated (bs-7166R) at 1:200 overnight at 4°C, DAB staining.