bs-7416R [Primary Antibody]
DNA polymerase delta Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DNA polymerase delta

Immunogen Range: 101-200/469


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5425

Source: KLH conjugated synthetic peptide derived from human DNA polymerase delta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). These exonucleases include the family of DNA polymerases (3). DNA pol , _, ∂, and e are involved in DNA replication and repair (4). DNA pol ∂ and DNA pol e are multisubunit enzymes, with DNA pol ∂ consisting of two subunits p125, which interacts with the sliding DNA clamp protein PCNA, and p50 (5). The nuclear-encoded DNA pol © is the only DNA polymerase required for the replication of the mitochondrial DNA (6). DNA pol is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis (7). DNA pol œ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks (8).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with DNA polymerase delta Polyclonal Antibody, Unconjugated (bs-7416R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Lane 1: Mouse Skin tissue lysates; Lane 2: Mouse Large intestine tissue lysates; Lane 3: Mouse Testis tissue lysates probed with DNA polymerase delta Polyclonal Antibody, Unconjugated (bs-7416R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.