bs-7464R [Primary Antibody]
Smad2 (Ser250) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Smad2 (Ser250)

Modification Site: Ser250

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4087

Swiss Prot: Q15796

Source: KLH conjugated synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser250

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse skin lysates probed with Smad2 (Ser250) Polyclonal Antibody, Unconjugated (bs-7464R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.