Host: Rabbit
Target Protein: VG5Q
Immunogen Range: 461-560/714
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 55109
Swiss Prot: Q8N302
Source: KLH conjugated synthetic peptide derived from human VG5Q/AGGF1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 81
Cross Reactive Species:
Mouse
Others
Predicted Cross Reactive Species:
Human
Rat
Dog
Cow
Pig
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: mouse lung lysates Lane 2: mouse intestine lysates probed with Anti VG5Q/AGGF1 Polyclonal Antibody, Unconjugated (bs-7509R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 79kD. Observed band size: 79kD.
Antigen: bs-7509P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in Microplate Reader by 450nm\n