bs-7525R
[Primary Antibody]
Tenomodulin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: TNMD
Immunogen Range: 151-250/317
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 64102
Swiss Prot: Q9H2S6
Source: KLH conjugated synthetic peptide derived from human TNMD
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Tenomodulin is related to chondromodulin-I, which is a cartilage-specific glycoprotein that functions to stimulate chondrocyte growth and to inhibit tube formation of endothelial cells. This protein is also an angiogenesis inhibitor. Genetic variation in this gene is associated with a risk for type 2 diabetes, central obesity and serum levels of systemic immune mediators in a body size-dependent manner. This gene is also a candidate gene for age-related macular degeneration, though a direct link has yet to be demonstrated.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 37
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.Read more>>
- Wada, Satoshi, et al. "H3K9MTase G9a is essential for the differentiation and growth of tenocytes in vitro." Histochemistry and Cell Biology (2015): 1-8.Read more>>
- Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.Read more>>
- Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.Read more>>
- Shuai Chen. et al. Tenogenic adipose-derived stem cell sheets with nanoyarn scaffolds for tendon regeneration. Mat Sci Eng C-Mater. 2021 Feb;119:111506Read more>>
- Chen Guanyin. et al. Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits. Stem Cells Int. 2020;2020:8822609Read more>>
- Kai KANG. et al. Lrrc32 accelerates tenogenic differentiation of tendon-derived stem cells and promotes Achilles tendon repair in rats. EXP ANIM TOKYO. 2022 Aug 05Read more>>
- Kang Lu. et al. Micropattern Silk Fibroin Film Facilitates Tendon Repair In Vivo and Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells through the 21/FAK/PI3K/AKT Signaling Pathway In Vitro. STEM CELLS INT. 2023 Jan 13;2023:2915826Read more>>
- Han Wang. et al. Introduction of tenomodulin by gene transfection vectors for rat bone tissue regeneration. REGEN THER. 2023 Mar;22:99Read more>>
- Lu, Kang. et al. N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin 5/1/PI3K/AKT signaling. BMC MOL CELL BIOL. 2023 Dec;24(1):1-13Read more>>
- Xin Wang. et al. Irreversible Electroporation Improves Tendon Healing in a Rat Model of Collagenase-Induced Achilles Tendinopathy. AM J SPORT MED. ;()Read more>>
- Ximin Yuan. et al. Tough Gelatin Hydrogel for Tissue Engineering. Advanced Science. 2023 Jun;:2301665Read more>>
- Zhirui Dong. et al. Tendon Repair and Regeneration Using Bioinspired Fibrillation Engineering That Mimicked the Structure and Mechanics of Natural Tissue. ACS NANO. 2023;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti TNMD/tenomodulin Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin-embedded Rat Stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Tenomodulin Polyclonal Antibody, Unconjugated (bs-7525R) at 1:400 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Lane 1: Rat Skeletal muscle lysates; Lane 2: Mouse Skeletal muscle lysates probed with TNMD/Tenomodulin Polyclonal Antibody, Unconjugated (bs-7525R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat muscle lysates; Lane 2: HepG2 cell lysates; Lane 3: Rat Heart lysates; Lane 4: SW480 cell lysates probed with TNMD/Tenomodulin Polyclonal Antibody, Unconjugated (bs-7525R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tenomodulin) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tenomodulin) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tenomodulin) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tenomodulin) Polyclonal Antibody, Unconjugated (bs-7525R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.