Host: Rabbit
Target Protein: Creatine Kinase MM
Immunogen Range: 221-330/381
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1158
Swiss Prot: P06732
Source: KLH conjugated synthetic peptide derived from human Creatine Kinase MM
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 43
Cross Reactive Species:
Mouse
Rat
Zebrafish
Predicted Cross Reactive Species:
Human
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Rueda, Elda M., et al. "The cellular and compartmental profile of mouse retinal glycolysis, tricarboxylic acid cycle, oxidative phosphorylation, and~ P transferring kinases." Molecular Vision 22 (2016):847-885 .Read more>>
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Creatine Kinase MM Polyclonal Antibody, Unconjugated (bs-7530R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Skeletal Muscle Lysates; Lane 2: Mouse Heart Lysates; Lane 3: Rat Skeletal Muscle Lysates; Lane 4: Rat Heart Lysates. Probed with Creatine Kinase MM polyclonal Antibody, unconjugated (bs-7530R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.