bs-7551R [Primary Antibody]
TNFAIP3 interacting protein 3 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNFAIP3 interacting protein 3

Immunogen Range: 51-150/325


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7128

Source: KLH conjugated synthetic peptide derived from human TNFAIP3 interacting protein 3/ABIN3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

ABIN-3 is a member of the A20-binding inhibitor of NF-kappaB activation (ABIN) protein family. Similar to the previously characterized human ABINs (ABIN-1 and ABIN-2), ABIN-3 can bind to A20 and inhibit NF-kappaB activation. In contrast, mouse ABIN-3 is incapable of inhibiting NF-kappaB activation by proinflammatory stimuli because the protein lacks a complete ABIN homology domain, which is required for the funcitonal activity of human ABIN-3.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Adrenal gland tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Spleen tissue lysates; Lane 6: Rat Adrenal gland tissue lysates; Lane 7: Rat Kidney tissue lysates probed with TNFAIP3 interacting protein 3 Polyclonal Antibody, Unconjugated (bs-7551R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TNFAIP3 interacting protein 3) Polyclonal Antibody, Unconjugated (bs-7551R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.