bs-7555R [Primary Antibody]
EPH A3+A4+A5 (Tyr779) Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: EPH A3+A4+A5 Tyr779

Modification Site: Tyr779

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2042

Source: KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of EPH 3 Tyr779

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

EphA3 is a member of the Ephrin (Eph) family. The Ephrins and Eph-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the Ephrin A (EphA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the Ephrin B (EphB) class, which are transmembrane proteins. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 108


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human HeLa cell lysates; Lane 2: Human HeLa(Serum-starve cells overnight, treat with the freshly made Pervanadate for 20min at 37°C.) probed with phospho-EPH A3+A4+A5 ( Tyr779) Polyclonal Antibody, Unconjugated (bs-7555R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.