bs-75992C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at +4°C.

Product Information:

CAS Number: 19088-73-4

Molecular formula: C10H5NO3

Molecular weight: 187.15

Purity: >98% (TLC)

Appearance: Light red powder.

Solubility: Soluble in alcohols or DMF.

InChiKey: IJQYTHQDUDCJEQ-UHFFFAOYSA-N

SMILES: OC1=CC=C2C=C(C#N)C(=O)OC2=C1

Description:

Phenolic dyes with high pKas - such as 7-hydroxycoumarin (often called umbelliferone) and the more common 7-hydroxy-4-methylcoumarin - are not fully deprotonated and therefore not fully fluorescent unless the pH of the reaction mixture is raised to above pH ~10. Thus, substrates derived from these fluorophores are seldom used for continuous measurement of enzymatic activity in solution or live cells. The similar 3-cyano-7-hydroxycoumarin and 6,8-difluoro-7-hydroxy-4-methylcoumarin have lower pKas, making them suitable for a broader range of applications. Moreover, the -trifluoromethylumbelliferone product has longer-wavelength spectra and stronger absorbance than -methylumbelliferone. Ether, ester and phosphate substrates derived from these phenolic dyes may be fluorescent but invariably exhibit shorter-wavelength absorption and emission spectra.