DATASHEET
Formulation
Powder
Storage:
Stable for 2 years after receipt when stored at +4°C.
Product Information:
CAS Number: 124387-19-5
Molecular formula: C25H16O9
Molecular weight: 460.39
Purity: >90% (HPLC)
Appearance: White powder.
Solubility: Soluble in DMSO.
InChiKey: DCPDRLHHNBUQPJ-UHFFFAOYSA-N
SMILES: CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2
Description:
CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.