DATASHEET
Formulation
Powder
Storage:
Stable for 2 years after receipt when stored at +4°C.
Product Information:
CAS Number: 136832-63-8
Molecular formula: C25H17ClO7
Molecular weight: 464.85
Purity: >95% (HPLC)
Appearance: Off-white to yellow powder.
Solubility: Soluble in DMSO.
InChiKey: IPJDHSYCSQAODE-UHFFFAOYSA-N
SMILES: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(CCl)=C3)C1=CC=C(OC(C)=O)C=C1O2
Description:
Thiol-reactive, cell permeant green fluorescent probe with high selectivity (Ex/Em: 492/517nm). This fluorescent dye is well suited for monitoring cell movement or location. Freely passes through cell membranes, where it is transformed into cell membrane-impermeant reaction products and is converted to the highly fluorescent probe (fluorescein) once the acetate groups are cleaved by intracellular esterases. The dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells and brightly fluorescent at physiological pH. The green excitation/emission spectra are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.