DATASHEET
Formulation
Solid
Storage:
Stable for 2 years after receipt when stored at -20°C.
Product Information:
CAS Number: 76877-33-3
Molecular formula: C24H22N2O4
Molecular weight: 402.44
Purity: >95% (HPLC)
Appearance: Yellow solid.
Solubility: Soluble in DMSO, DMF, acetonitrile or chloroform.
InChiKey: YGIABALXNBVHBX-UHFFFAOYSA-N
SMILES: CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O
SMILES: CCN(CC)C1=CC2=C(C=C1)C(C)=C(C(=O)O2)C1=CC=C(C=C1)N1C(=O)C=CC1=O
Description:
Widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.