bs-76133C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at +4°C.

Product Information:

CAS Number: 2044-85-1

Molecular formula: C24H14Cl2O7

Molecular weight: 485.27

Purity: ≥97% (HPLC)

Appearance: White to off-white powder.

Solubility: Soluble in DMSO or acetone.

InChiKey: VQVUBYASAICPFU-UHFFFAOYSA-N

SMILES: CC(=O)OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C22OC(=O)C3=C2C=CC=C3)=C1

SMILES: CC(=O)OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C22OC(=O)C3=CC=CC=C23)=C1

Description:

A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2’,7’-dichlorofluorescein (DCF). Spectral data: lambdaex=504nm, lambdaem=524nm in 0.1 M Tris pH 8.0 (esterase).